Year: 2019 | Month: June | Volume 9 | Issue 3
A Study on the Molecular Epidemiology of Brucella abortus among Cattle in Western Rajasthan
Abstract:
Bovine brucellosis is usually caused by biovars of Brucella abortus. It is one of the important diseases impacting the dairy sector in India, including Rajasthan, which is often neglected because of its endemicity and often persists in the poorest and most vulnerable populations. The present study was conducted to assess the prevalence of Brucella abortus in the cases of reproductive disorders such as abortion, stillborn or weak calves, neonatal mortality, retained placenta, delayed conception and infertility among the cattle in Western Rajasthan. A total of 87 vaginal samples including swabs (64) and discharges (23) were collected from cattle with a clinical history of reproductive failures, and screened using Brucella genus specific bcsp31 gene based PCR followed by Brucella abortus species specific IS711 based PCR. The results revealed 12.64% of the clinical samples positive for the presence of Brucella spp., including 12.50% of vaginal swabs and 13.04% of the vaginal discharges. All the samples detected positive in genus specific PCR were detected positive for the presence of Brucella abortus. The present study confirmed the considerable involvement of Brucella abortus as a major etiological agent in the clinical cases of reproductive disorders in cattle highlighting the urgent need for implementation of strict measures to control the disease in Western Rajasthan.
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